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The restriction enzymes AgeI and AvaI recognition sites are shown below. (NOTE, the arrows represent where the enzymes actually cut the DNA for each strand). AgeI: 5’-ACCGGT-3’ AvaI: 5’-CCCGGG-3’...

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The restriction enzymes AgeI and AvaI recognition sites are shown below. (NOTE, the arrows represent where the enzymes actually cut the DNA for each strand).


AgeI: 5’-ACCGGT-3’ AvaI: 5’-CCCGGG-3’

3’-TGGCCA-5’ 3’-GGGCCC-5’


Sequence 1: 5’-GGACCGGTCC-3’ Sequence 2: 5’-AACCCGGGTT-3’

3’-CCTGGCCAGG-3’ 3’-TTGGGCCCAA-5’

A. Write out the DNA fragments that are formed after cutting the above DNA sequences with:

Sequence I with AgeI :

Sequence 2 with AvaI:

Answered Same Day Oct 16, 2021

Solution

Riyanka answered on Oct 17 2021
171 Votes
The restriction enzymes AgeI and AvaI recognition sites are shown below. (NOTE, the a
ows represent where the enzymes actually cut the DNA for each strand
AgeI: 5’-ACCGGT-3’ AvaI: 5’-CCCGGG-3’
3’-TGGCCA-5’ 3’-GGGCCC-5’
Sequence 1: 5’-GGACCGGTCC-3’ Sequence 2: 5’-AACCCGGGTT-3’
...
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