03/05/2022, 18:41 2022_Open-book assessment 1.
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2022_Open-book assessment 1.
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BIOL2184 – Gene technologies
Open-book assessment XXXXXXXXXXpoints, 30%)
This assessment reflects your industry-related research
Task: Genetic reprogramming of the valuable chemicals’ production in plants
2022_BIOL2184 Open-book assessment 1.pdf
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Deadline: May 8th 23:59
10% penalty for each extra day
Assessment
ief, criteria, and ru
ic.
This assignment outlines course learning outcomes (CLOs) requirements,
which you will use during your future industry-related experience.
On successful completion of this course, you should be able to:
CLO 1. Understanding science:
1.1. You will demonstrate a coherent understanding of biological sciences by articulating the
methods of science and explaining why cu
ent biological knowledge is both contestable and
testable through further inquiry.
1.2. You will demonstrate a coherent understanding of biological sciences by explaining the
ole and relevance of biological sciences in society.
CLO 2. Scientific knowledge:
2.1. You will exhibit depth and
eadth of knowledge by demonstrating a well-developed
understanding of biological sciences.
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2.2. You will exhibit depth and
eadth of knowledge by demonstrating that biotechnology has
interdisciplinary connections with other sciences.
CLO 3. Inquiry and problem-solving:
3.1. You will be able to critically analyse and solve problems in biotechnology by gathering,
synthesizing and critically evaluating information from a range of sources.
3.2. You will be able to critically analyse and solve problems in biotechnology by designing
and planning an investigation, including developing a testable hypothesis.
3.3. You will be able to critically analyse and solve problems in biotechnology by selecting and
applying practical and/or theoretical techniques with technical competence in conducting field,
laboratory-based, or virtual experiments.
3.4. You will be able to critically analyse and solve problems in biotechnology by collecting,
accurately recording, interpreting, and drawing conclusions from scientific data.
CLO 4. Communication: You will be an effective communicator of biological sciences by
effectively communicating scientific results, information, or arguments using a range of modes
(oral, written, visual) for a variety of purposes and audiences.
CLO 5. Personal and professional responsibility: You will be accountable for individual
learning and scientific work in biotechnology by being an independent and self-directed
learner.
Performance descriptors: For HD XXXXXXXXXX%) you need to achieve excellently all tasks following the instructions
and guidelines listed above
Criteria Ratings Pts
Understanding of
molecular cloning
techniques and thei
applications
100.0 Pts 50.0 Pts 25.0 Pts 0.0 Pts 100
Good response
Satisfactory
esponse
Unsatisfactory
esponse
No
esponse
provided
Appropriate, relevant
key points covered
Understanding the
task and the
information but not all
Not demonstrated
an understanding
of the topic
No
esponse
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Open-book Assignment 1
key points are
included
information ,
often inaccurate
or i
elevant
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Criteria Ratings Pts
20 pts
20 pts
20 pts
20 pts
Theoretical part
Application of
transgenic
technologies in
plant
iotechnology
and modern
agriculture
(appx 1-2
pages)
20 Pts
Excellent
The research topic
has been clearly
articulated and
explained. The
application of
transgenic
technologies in plant
iotechnology and
modern agriculture
has been answered
with related
eferences
10 Pts
Good
Most but not all parts of
the question have been
answered sufficiently.
The research topic has
een articulated but
may lack some clarity
or detail. A good to a
satisfactory number of
eferences has been
shown.
5 Pts
Brief answe
Most parts of the
question have not been
answered sufficiently. An
explanation of the
esearch topic is
inadequate or has not
een provided.
References and examples
concepts were shown,
ut some of them are
i
elevant.
0 Pts
Not
satisfactory
No attempt
has been
made to
satisfactorily
answer any
parts of the
question.
Research part
Design and
describe a
transgene fo
production of X
in leaves
(promoter,
coding region,
terminator). You
can design
more than one
transgene
20 Pts
Excellent
All parts of the question
have been clearly
articulated and explained:
Transgene and its key
components for the
production of X in leaves
were clearly explained.
The strategy is smart. The
application of key
transgene components
was clearly explained.
10 Pts
Good
Understanding the task and
the information but not all key
points are included. One o
two minor parts of the
question are unanswered. The
proposed strategy fo
transgene’s design is not the
most efficient. The application
of key transgene components
was not clearly explained.
5 Pts
Brief answe
Several parts
of the question
are
unanswered.
An appropriate
strategy has
een
proposed. Not
demonstrated
a good
understanding
of the topic.
0 Pts
Not
satisfactory
No attempt
has been
made to
satisfactorily
answer any
parts of the
question.
Research part
Design and
describe a
vector that
contains you
transgene(s)
20 Pts
Excellent
The research topic has been
clearly articulated and
explained. All parts of the
question have been
answered: Vector and its key
components for the
production of X in leaves
were clearly shown. The
strategy is smart. The
application of key transgene
components was clearly
explained.
10 Pts
Good
Understanding the task and
the information but not all
key points are included. One
or two minor parts of the
question are unanswered.
The proposed strategy fo
transgene’s design is not the
most efficient. The
application of key transgene
components was not clearly
explained.
5 Pts
Brief answe
Several parts
of the
question are
unanswered.
An appropriate
strategy has
een
proposed. Not
demonstrated
a good
understanding
of the topic.
0 Pts
Not
satisfactory
No attempt
has been
made to
satisfactorily
answer any
parts of the
question.
Research part
Design a
strategy fo
transformation,
generation, and
selection of the
transgenic
plants
20 Pts
Excellent
The research topic has
een clearly articulated
and explained. Design a
strategy for transformation,
generation, and selection
of the transgenic plants
were clearly explained. The
strategy is smart. The
application of key parts of
the project was clearly
explained.
10 Pts
Good
Understanding the task and
the information but not all key
points are included. One o
two minor parts of the
question are unanswered.
The proposed strategy fo
transformation, generation
and selection of the
transgenic plants is not the
most efficient and /or was not
clearly explained.
5 Pts
Brief answe
Several parts
of the question
are
unanswered.
An appropriate
strategy has
een
proposed. Not
demonstrated
a good
understanding
of the topic.
0 Pts
Not
satisfactory
No attempt
has been
made to
satisfactorily
answer any
parts of the
question.
03/05/2022, 18:41 2022_Open-book assessment 1.
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Total points: 100
Criteria Ratings Pts
20 pts
Research part
Design
strategies to
confirm you
generated
transgenic plant
20 Pts
Excellent
The research topic has been
clearly articulated and
explained. Design a strategy
to confirm your generated
transgenic plant was clearly
explained. The strategy is
smart. The application of
key parts of the project was
clearly explained.
10 Pts
Good
Understanding the task
and the information but
not all key points are
included. One or two
minor parts of the
question are unanswered.
The proposed strategy to
confirm transgenic plants
was not clearly explained.
5 Pts
Brief answe
Several parts of
the question are
unanswered. An
appropriate
strategy has
een proposed.
Not
demonstrated a
good
understanding of
the topic.
0 Pts
Not
satisfactory
No attempt
has been
made to
satisfactorily
answer any
parts of the
question.
RMIT Classification: Trusted
BIOL2184 – Gene technologies
Open-book assessment XXXXXXXXXXpoints, 30%)
Open-book assessment 1
This assessment reflects your routine industry-related research:
Task: Genetic reprogramming of the production of the valuable
chemical in plants
Project
Your company grows plants that produce a valuable chemical X in floral petals (flowers).
Genes responsible for the biosynthesis of this chemical are GeneXA, GeneXB,
and GeneXC. The petal-specific transcription factor, TFX, regulates these genes.
Problem: These plants cannot be commercially used for the large-scale production
of X because:
• they do not produce many flowers;
• flowers are small and
• the production is seasonal and short since these plants flower only in
spring and flowering lasts just a few days.
As a result, it is not economical to produce enough biomass of petals for the
commercial production of chemical X.
Your project
To increase the production of the chemical X, your company decided to reprogram
the expression of genes XA, XB and XC, re-directing the production of chemical X
from flowers to leaves of the same plants. The rationales behind this decision are:
• these plants have big leaves,
• leaves’ growth is not seasonal and
• leaves and flowers have the same genome, and all genes involved
in X production exist in leaves but are not active.
RMIT Classification: Trusted
You are the lucky researcher who must run this project. You need to design a
strategy to conduct this project.
You need to:
1. Theoretical part:
Describe an application of transgenic technologies in plant biotechnology and
modern agriculture with examples (appx 1-2 pages): 20 marks
Appx 3 examples.
• Why are these papers interesting?
• What has been achieved?
• How?:
iefly experimental part
2. Research part:
• Design transgene(s) for the production of X chemical in leaves (e.g. promoter,
coding region, terminator). Show a transgene as a map: 20 marks
• Design and describe a vector that contains your transgenes. The vector
should have a selectable marker for plants. Show vector as a map: 20 marks
• Design a strategy for transformation, generation, and selection of the
transgenic plants: 20 marks
• Design strategies confirm your generated transgenic plant: expression of the
transgene and the production of chemical X: 20 marks
Your report:
A 1000+ word review. You can add pictures and maps if required. Use paragraphs
to separate different parts of the review. Show in bold critical components of the
gene silencing mechanisms.
You must clearly address a research topic ● The information presented must
e accurate and logically organised ● You should demonstrate an innovative
solution to an