Scoring for Lab 5:
Screen shot – 10 points
Activity Data Code – 5 points
Data Tables – 15 points
Question Completion – 20 points
Random Questions Scored – 50 points
· On your own and without assistance, complete this Lab Activity Form electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus).
· Answer ALL questions in your own words. If a direct quote is needed, put the words in quotation marks and cite the source.
· To conduct the laboratory exercises, use the Table Top Science link located under Course Content. Read the background information and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.
· Students should submit the document as a Word (.doc or .docx) or PDF file to the assignment folder for best compatibility.
· If screen shots are missing, the lab earns 0 points until it is included.
Data Sheet: Activity - Photosynthesis and Respiration
All Content is Copyright Protected and May NOT Be Posted or Shared Outside Of The Classroom
Name
Course
Date
Activity Data Code
Take a screenshot of your results. Insert the picture here:
Net photosynthesis = Photosynthesis – Respiration
Net ca
on gain = Ca
on gain in photosynthesis – Ca
on loss in respiration
Rate = change
XXXXXXXXXXtime interval
Procedure I - Net Photosynthesis Rates - 100% Illumination
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
O2 Rate = change in oxygen concentration
XXXXXXXXXXtime interval
CO2 Rate = change in ca
on dioxide concentration
XXXXXXXXXXtime interval
2
TableTop Science – All Rights Reserved
1
TableTop Science – All Rights Reserved
Note: ppm stands for parts-per-million and is a unit for measuring concentration
Leaf
Sample
Type
Illumination
Level
Oxygen
Concentration
Change Data (ppm)
Elapsed
Time
(s)
Oxygen
Change Rate
(ppm/s)
Sun-
Adapted
100%
Shade-Adapted
100%
Leaf
Sample
Type
Illumination
Level
Ca
on Dioxide
Concentration
Change Data
(ppm)
Elapsed
Time
(s)
Ca
on Dioxide
Change Rate
(ppm/s)
Sun-
Adapted
100%
Shade-Adapted
100%
Observations and Questions
[1] How is chlorophyll involved during photosynthesis?
[2] A. What are enzymes?
B. What role do they play in biological and chemical reactions?
[3] How might differences in enzyme concentrations affect rates of photosynthesis and respiration?
[4] Show your oxygen change rate calculation for the Sun-Adapted data run.
[5] Show your ca
on dioxide change rate calculation for the Shade-Adapted data run.
[6] Examine YOUR results.
A. Do your results reveal a possible relationship between (rates of) production of oxygen and ca
on dioxide? If so, what is the relationship?
B. Explain why there is or is not a relationship. Include an example of your results as part of your explanation.
[7] A. What does cu
ent research say is different about plants with shade-adapted leaves and sun-adapted leaves regarding differences in chlorophyll production and enzyme activity? (use your own words!)
B. Are your results for Procedure I consistent with these research findings? Yes or No?
C. Explain how your results are or are not consistent with the research. Include an example of your results from Procedure I to support your explanation.
[8] How is the net photosynthesis rate related to the rates YOU determined? (note that this is NOT a calculation)
[9] Explain why (or why not) you would expect cellular respiration to occur when the illumination is 100%?
Procedure II – Cellular Respiration Rates - 0% Illumination Data Run
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
O2 Rate = change in oxygen concentration
XXXXXXXXXXtime interval
CO2 Rate = change in ca
on dioxide concentration
XXXXXXXXXXtime interval
Note: ppm stands for parts-per-million and is a unit for measuring concentration
4
TableTop Science – All Rights Reserved
3
TableTop Science – All Rights Reserved
Leaf
Sample
Type
Illumination
Level
Oxygen
Concentration
Change Data (ppm)
Elapsed
Time
(s)
Oxygen
Change Rate
(ppm/s)
Sun-
Adapted
0%
Shade-Adapted
0%
Leaf
Sample
Type
Illumination
Level
Ca
on Dioxide
Concentration
Change Data (ppm)
Elapsed
Time
(s)
Ca
on Dioxide
Change Rate
(ppm/s)
Sun-
Adapted
0%
Shade-Adapted
0%
Observations and Questions
[10] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.
[11] Sample Calculation: Show your ca
on dioxide change rate calculation for the Shade-Adapted data run.
[12] A. Include an example of your results for the change in ca
on dioxide concentration as a reference and compare the cellular respiration rates for sun-adapted and shade-adapted leaves.
B. Explain the biological principles
ases underlying this comparison.
[13] A. What would be the photosynthesis rate if you measured it at 0% illumination in Procedure II?
B. Explain how you a
ived at your answer. Include an example of your results your results from Procedure I to assist with your explanation.
Procedure III - Low-Light Net Photosynthesis Rates - 15% Illumination
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
O2 Rate = change in oxygen concentration
XXXXXXXXXXtime interval
CO2 Rate = change in ca
on dioxide concentration
XXXXXXXXXXtime interval
Note: ppm stands for parts-per-million and is a unit for measuring concentration
Leaf
Sample
Type
Illumination
Level
Oxygen
Concentration
Change Data (ppm)
Elapsed
Time
(s)
Oxygen
Change Rate
(ppm/s)
Sun-
Adapted
15%
Shade-Adapted
15%
6
TableTop Science – All Rights Reserved
7
TableTop Science – All Rights Reserved
Leaf
Sample
Type
Illumination
Level
Ca
on Dioxide
Concentration
Change Data
(ppm)
Elapsed
Time
(s)
Ca
on Dioxide
Change Rate
(ppm/s)
Sun-
Adapted
15%
Shade-Adapted
15%
Observations and Questions
[14] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.
[15] Most leaves spend time in different concentrations of illumination throughout the day and throughout the year. Based on the data you collected in Procedures I (100% illumination) and III (15% illumination), which system of leaves produces the most oxygen overall?
A. Sun-adapted or Shade-adapted? _________
B. Explain how your results support your answer. How did you determine whether the answer was sun-adapted or shade adapted leaves? What process did you use? Include an example of your results in the explanation.
[16] A. What does cu
ent research say is different about plants with shade-adapted leaves and sun-adapted leaves regarding differences in chlorophyll production and enzyme activity? (use your own words!)
B. Are your results for Procedure II and Procedure III consistent with these research findings? Yes or No?
C. Explain how your results are or are not consistent with the research. Include an example of your results from Procedures II and III to support your explanation.