Scoring for Lab 4:
Screen shot – 10 points
Activity Data Code – 5 points
Data Tables – 15 points
Question Completion – 20 points
Random Questions Scored – 50 points
· On your own and without assistance, complete this Lab Activity Form electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus).
· Answer ALL questions in your own words. If a direct quote is needed, put the words in quotation marks and cite the source.
· To conduct the laboratory exercises, use the Table Top Science link located under Course Content. Read the background information and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.
· Students should submit the document as a Word (.doc or .docx) or PDF file to the assignment folder for best compatibility.
· If screen shots are missing, the lab earns 0 points until it is included.
Data Sheet: Activity - Enzymes
All Content is Copyright Protected and May NOT Be Posted or Shared Outside Of The Classroom
Name
Course
Date
Activity Data Code
Take a screenshot of your results. Insert the picture here:
Procedure I - Enzyme Rates - Concentration Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
Rate = Change = ppt
Time Interval XXXXXXXXXXs
2
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1
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Note: ppt stands for parts-per-thousand and is a unit for measuring concentration.
Trial
Concentration
Level of Enzyme
Oxygen
Concentration
Change Data (ppt)
Elapsed
Time
(s)
Enzyme
Rate
(ppt/s)
1
2.5%
20.0
2
5.0%
20.0
3
10%
20.0
Observations and Questions
[1] Sample Calculation: Show your enzyme rate calculation for Trial 1. SHOW YOUR WORK
[2] Convert enzyme concentrations of 2%, 6.5%, and 15% to ppt units.
A. 2.0% = ______ ppt
B. 6.5% = ______ ppt
C.15.0% = _____ ppt
[3] For Procedure I/Table I, identify:
A. Dependent variable:
B. Independent variable:
C. 2 or more Controlled variables:
[4] Draw a line graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis, units, and provide an appropriate title.
Procedure II - Enzyme Rates - Temperature Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
Rate = Change = ppt
Time Interval XXXXXXXXXXs
2
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3
TableTop Science – All Rights Reserved
Note: ppt stands for parts-per-thousand and is a unit for measuring concentration.
2
TableTop Science – All Rights Reserved
3
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Trial
Temperature of Enzyme
(C)
Oxygen
Concentration
Change Data (ppt)
Elapsed
Time
(s)
Enzyme
Rate
(ppt/s)
1
20.0
20.0
2
30.0
20.0
3
55.0
20.0
Observations and Questions
[5] Sample Calculation: Show your enzyme rate calculation for Trial 1. SHOW YOUR WORK
[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.
Procedure III - Enzyme Rates - pH Dependence
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
Rate = Change = ppt
Time Interval XXXXXXXXXXs
Note: ppt stands for parts-per-thousand and is a unit for measuring concentration.
Trial
pH
Level of Enzyme
Oxygen
Concentration
Change Data (ppt)
Elapsed
Time
(s)
Enzyme
Rate
(ppt/s)
1
6.0
20.0
2
7.0
20.0
3
9.0
20.0
Observations and Questions
[7] Sample Calculation: Show your enzyme rate calculation for Trial 1. SHOW YOUR WORK
[8] Draw a line graph for Procedures II and III. Label the axis, units, and provide an appropriate title. Plot the data for each Procedure on a separate plot.
[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.
[10] What conclusions can be drawn about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?
[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.
Factor Chosen:
Explanation:
4
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5
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