PowerPoint Presentation
Guidelines on writing the mini project report
Identification of an Unknown microorganism
BIOL2416 & BIOL2417
Diagnostic Microbiology
❑ To understand the importance of identification of bacteria and
its relevance to applied microbiology today.
❑ To be able to use the phenotypic tests learnt in previous
practical sessions to identify bacterial isolates to species level
using the RMIT identification tables.
❑ To understand the basis of the biochemical reactions tests and
what they tell us about the metabolism of the investigated
acterium.
Reading:
● Textbook: Microbiology with Diseases by Body System, 14th or
15th edition, Pearson.
● Any relevant peer-reviewed papers published in reputable
journals.
Learning Objectives
FORMAT
➢Introduction and Aims
➢Materials and Methods
➢Table of Results (Deductive reasoning)
➢Discussion
➢References
➢Scanned results sheets and flowchart
Word Limits
BIOL2416 Undergraduates : XXXXXXXXXX% Excluding Tables,
eference list, worksheet and flowchart.
BIOL2417 Postgraduates: XXXXXXXXXX% Excluding Tables,
eference list, worksheet and flowchart.
The Report Format
In the introduction, provide the background information about
this project including the following information:
The importance and the benefits of identifying
microorganisms.
Highlight some rapid methods and automated equipment
cu
ently used in identifying unknown microbes.
The aim of the project:
• What did you intend to investigate in this study?
• What did you expect to find out in this study?
• What was the method(s) or approach(es) used in this study?
Introduction
What information is required in Materials and Methods?
TABULATE the list of biochemical tests and media used in
identifying your unknown. The table must have an appropriate title
legend.
Unknown Organism (code no.), growth medium, and growth
conditions?
Full name of the biochemical tests and key reagents used for
each test with expected observations. (USE GERMM FOR THIS
SECTION and include citation)
Growth Media and incubation conditions – i.e. time, temperature
and gaseous conditions (aerobic/ anaerobic).
DO NOT include the following in Materials and Methods:
Laboratory equipment like Bunsen burners, loops, swabs etc.
The detailed method of each biochemical test. Just cite GERMM
Any Results from your test
Materials and Methods
Materials and Methods- in text citation
Citation: G.E.R.M.M and RMIT Practical Manual according to
RMIT APA referencing.
Course materials are not required in reference list. Please refer
to the weblink for more information
https:
www.lib.rmit.edu.au/easy-cite
• Diagnostic Microbiology Workbook’s author is RMIT
Microbiology Teaching Team.
• GERMM’s author is RMIT Microbiology Teaching Team
https:
www.lib.rmit.edu.au/easy-cite
A short paragraph on the identity of your unknow organism.
E.g. An unknown No “7” was identified as ”Staphylococcus aureus”. The unknown grew on
HBA … and MAC… and colony morphology was.., catalase was .. Gram staining and
microscopy techniques confirmed that this is a XXXXXXXXXXGram reaction, and bacterial cell
shape). Furthermore, the identity of this unknown bacterium, as assessed by biochemical
tests indicated in the table below (or Table no.) was confirmed to be XXXXXXXXXXGenus-
species). The RMIT ID Table number used for identifying your unknown must be
included here.
Deductive reasoning: Based on the flowchart,
iefly explain
how differential biochemical test results, and/or observation of
the growth on a differential medium contributed to the final
esult to identify the unknow bacterium through genus to species
levels, i.e. how did the test/growth results eliminate other
genera and species, leading you to find the identity of you
unknown bacterium. CITE “RMIT ID Tables for Bacteria”
Explain any unexpected results obtained in identification of your
unknown bacterium. E.g. if a lactose fermenter gave you a
negative result for lactose fermentation. Why it happened? And
suggest what should you do to verify it.
Results
Do not repeat your results here.
In your discussion, select one clinical case from the literature
where this bacterium was reported to be isolated. What was the
clinical case? Name of disease and main symptoms.
Use this clinical case as your starting point for discussion. Include
the following information in the discussion:
• What was the detection method used in the selected reference?
• Compare the accuracy and sensitivity of your method to theirs.
Furthermore:
• BIOL2416 UG – You need to cite 2 additional resources (journal
papers or authentic health authority cites) which reported clinical
cases caused by this bacterium. Name the disease, and relevant
ody system/organ.
• BIOL2417 PG– You need to cite 4 additional resources (journal
papers or authentic health authority cites) which reported clinical
cases caused by this bacterium. Name the disease, and relevant
ody system/organ.
Discussion
❏ Make sure you include in-text citation co
ectly – refer to APA
eferencing guide.
http:
www1.rmit.edu.au/li
ary
eferencing-guides
❏ “No Numbering” listing in APA referencing list.
❏ Do not include any images that are subject to copyright.
❏ Plagiarism will be dealt with according to RMIT plagiarism policy
and procedures.
References
http:
www1.rmit.edu.au/li
ary
eferencing-guides
Documents for submission:
A single pdfs file which includes the following combined
documents:
1. The full final written report +
2. A scanned copy of your signed results record +
3. A scanned copy of your final signed flowchart
Due Date :
BIOL2416: 23 Oct 2020, 23.59 pm
BIOL2417: 1 Nov 2020, 23.59pm
Scientific Report Submission:
• This mini project grades include your hands on and bacterial
identification techniques (RTC day 3-5), The written Scientific
Report, the Recorded results sheets and flowchart
• It is worth a total of 20% of the 50% marks allocated to the practical
component hurdle of this course.
The mini project assignment will be marked out of total 200
marks as explained below:
1. Your lab work (60 marks) & the 1st draft of your flow chart (40
marks). This grading will be completed by your demonstrator
during the lab session =100 marks.
2. Final written report + final Flow chart = 100 marks
Note: (Grading scheme detailed in the next two slides)
Marking Scheme
Marking Scheme: Lab work and 1st Flow Chart
(100 marks)
In class assessment by your demonstrato
A. Lab work (60 marks)
Criteria of assessment:
Microbiology practical techniques (20 marks)
Analytical thinking skill (20 marks)
Organization of work (20 marks)
B. Flow chart (40 marks)
Criteria of assessment:
Systematic, orderly flow of the identification steps (20 marks)
Differential tests included in each step (10 marks)
Expected genera/species are included in each step (10 marks)
RMIT University© School/Department/Area 12
Marking Scheme: Final Written Report (100 marks)
RMIT University© School/Department/Area 13
Scientific Report Sections Allocated Marks
Introduction and Aim 15
Materials and Methods 15
Results 20
Final flow chart & working lab sheet 10
Discussion 20
References/In text citation (relevant
eferences & co
ect format)
10
Gramma
Format 10
TOTAL 100
For inquiries on this assignment:
● Inquiries about the lab work and unknown identification
during the lab session: Ask your demonstrato
● General inquiries in person after the lab session: Talk to
Dr. Foong Yong (Stacey).
● Inquiries bout writing and submitting the report:
Contact Dr. Istivan or Dr. Yong in person or by email.
Guidelines on writing the mini project report
Identification of an Unknown microorganism
BIOL2416 & BIOL2417�Diagnostic Microbiology
Learning Objectives
The Report Format
Introduction
Materials and Methods
Materials and Methods- in text citation
Results
Discussion
References
Scientific Report Submission:�
Marking Scheme
Marking Scheme: Lab work and 1st Flow Chart �(100 marks)
Marking Scheme: Final Written Report (100 marks)
For inquiries on this assignment:
Table 1: Characteristics of Unknown
TEST
Results/Observation
Gram stain
GPR, Cockeye
Growth of HBA
2-3 mm, grey, round, condensed, raised
Growth of MAC
1-2 mm, light pink, small circles, condensed, convex
Catalase
Positive
Oxidase
NA
Tube Coagulase
Negative
Slide Coagulase
Negative
Urease
Positive
DNAsc
Negative
R/S Novobiocin
Resistant
R/S Polymyxin B
Susceptible