Microsoft Word - Fermentation Worksheet(1).docxFermentation Rates using Different Caohydrate Food...

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Microsoft Word - Fermentation Worksheet(1).docx
Fermentation Rates using Different Ca
ohydrate
Food Sources
Table 1 – Cumulative CO2 volume for different
ca
ohydrates
Time
(min)
Glucose Water Sucrose Maltose Starch
Gas
level
(mL)
Cumul.
CO2
Volume
(mL)
Gas
level
(mL)
Cumul.
CO2
Volume
(mL)
Gas
level
(mL)
Cumul.
CO2
Volume
(mL)
Gas
level
(mL)
Cumul.
CO2
Volume
(mL)
Gas
level
(mL)
Cumul.
CO2
Volume
(mL)
0 0.1 Zero 0 Zero 0.1 Zero 0.2 Zero 0.1 Zero
XXXXXXXXXX0.2
XXXXXXXXXX 0.2
XXXXXXXXXX 0.2
XXXXXXXXXX 0.2
XXXXXXXXXX1 0.2
XXXXXXXXXX4 0.2
Use the data from Table 1 to create Graph 1. Using Excel do a line of best fit
for each data set and have the equation for that line show on the graph.
Take the slope of each line, “m” in y=mx+b and put it in Table 2.
Insert calculations for fermentation rates here:
Table 2 – Fermentation Rates for Different Ca
ohydrates
Ca
ohydrate Type Fermentation Rate (mL CO2/min)

Use the data from Table 2 to create Graph 2.
Analyzing the Data
• Graph the cumulative CO2 volume data with time in minutes. Graph all
5 data sets on Graph 1. (Remember to use TAILS.)
• The fermentation rate (in ml CO2 per minute) can be determined by
calculating the slope of the line.
Use Excel to determine the line of best fit through your data
• Calculate the slope of each line to determine fermentation rate for
each ca
ohydrate and distilled water.

• Once the fermentation rate for each ca
ohydrate is determined record
the data in Table 2 and make a graph comparing fermentation rate vs
ca
ohydrate type.
Fill in the tables above and insert graphs 1 and 2 here:
What is your overall hypothesis for the experiment?
Does the data support or disprove your hypothesis?
If your data doesn’t support your hypothesis, what would be an alternative
hypothesis that is supported by the data?
Adapted from:
Reinking, L.N., Reinking, J.L. and K.G. Miller 1994. Fermentation, respiration
and enzyme specificity: A simple experiment with yeast. The American
Biology Teacher, 56:164-168.
Bonner, J.M XXXXXXXXXXA study of fermentation by Saccharomyces cerevesiae.
Workshop of the Association for Biology Laboratory Education, McDaniel
College, Westminster, MD.

XXXXXXXXXX:34

fermentation-worksheet1-0f0brguf.pdf 2021-03-08-06-34-xcuyqbky.pdf

Shweta · Accepted Answer

Sheet1
				Glucose			Time(min)	Gas level
			Time(min)	Gas level	Cumul. CO2Vol(ml)		0	0.1
			0	0.1	0		2	0.7
			2	0.7	0		4	2.5
			4	2.5	2.4		6	4.7
			6	4.7	4.6		8	5.2
			8	5.2	5.1		10	6.6
			10	6.6	6.5		12	7.6
			12	7.6	7.5
							Slope	0.6607142857
							Intercept	-0.05
				Water 			Time(min)	Gas level
			Time(min)	Gas level	Cumul. CO2Vol(ml)		0	0
			0	0	0		2	0
			2	0	0		4	0.1
			4	0.1	0.1		6	0.1
			6	0.1	0.1		8	0.1
			8	0.1	0.1		10	0.1
			10	0.1	0.1		12	0.1
			12	0.1	0.1
							Slope	0.0089285714
							INTERCEPT	0.0178571429
				Sucrose 
			Time(min)	Gas level	Cumul. CO2Vol(ml)		Time(min)	Gas level
			0	0.1	0		0	0.1
			2	0.7	0.6		2	0.7
			4	2.4	2.3		4	2.4
			6	3.5	3.4		6	3.5
			8	5	4.9		8	5
			10	6.2	6.1		10	6.2
			12	7.3	7.2		12	7.3
							slope	0.6285714286
							intercept	-0.1714285714
				Maltose			Time(min)	Gas level
			Time(min)	Gas level	Cumul. CO2Vol(ml)		0	0.2
			0	0.2	0		2	0.3
			2	0.3	0.1		4	0.5
			4	0.5	0.3		6	0.9
			6	0.9	0.7		8	1.4
			8	1.4	1.2		10	2.1
			10	2.1	1.9		12	2.4
			12	2.4	2.2
							slope	0.1982142857
							intercept	-0.075
				Starch
			Time(min)	Gas level	Cumul. CO2Vol(ml)		Time(min)	Gas level
			0	0.1	0		0	0.1
			2	0.2	0.1		2	0.2
			4	0.2	0.1		4	0.2
			6	0.2	0.1		6	0.2
			8	0.2	0.1		8	0.2
			10	0.2	0.1		10	0.2
			12	0.2	0.1		12	0.2
							slope	0.0053571429
							INTERCEPT	0.1535714286
Fermentation rate
Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	time
cumulative co2 vol (ml)
Fermentation rate
Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	time
cumulative co2 vol (ml)
Fermentation rate
Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	time
cumulative co2 vol (ml)
Fermentation rate
Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	time
cumulative co2 vol (ml)
Fermentation rate
Gas level	0	2	4	6	8	10	12	0.1	0.7	2.5	4.7	5.2	6.6	7.6	Gas level	0	2	4	6	8	10	12	0.

Microsoft Word - Fermentation Worksheet(1).docx Fermentation Rates using Different Carbohydrate Food Sources Table 1 – Cumulative CO2 volume for different carbohydrates Time (min) Glucose Water...

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