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Open-book assignment 2 (35%) This assessment contains two parts that reflect your industry-related activities: Part XXXXXXXXXXmarks) Research project report: appx 1000+ words, submission of word/PDF...

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Open-book assignment 2 (35%)

This assessment contains two parts that reflect your industry-related activities:

Part XXXXXXXXXXmarks)

Research project report:appx 1000+ words, submission of word/PDF document.
Generation of cut flowers (eg roses) with an extended shelf-life using genetic
engineering technologies

BIOL2184 Open-book assessment 2_Part 1.pdf

Deadline for submissionMay 29, 23:59

There will be 10% penalty for each extra submission day

Assessment brief, criteria, and rubric.
This assignment outlines course learning outcomes (CLOs) requirements,
which you will use during your future industry-related experience.
On successful completion of this course, you should be able to:
CLO 1. Understanding science:
1.1. You will demonstrate a coherent understanding of biological sciences by articulating the
methods of science and explaining why current biological knowledge is both contestable and
testable through further inquiry.
1.2. You will demonstrate a coherent understanding of biological sciences by explaining the
role and relevance of biological sciences in society.
CLO 2. Scientific knowledge:
2.1. You will exhibit depth and breadth of knowledge by demonstrating a well-developed
understanding of biological sciences.
2.2. You will exhibit depth and breadth of knowledge by demonstrating that biotechnology has
interdisciplinary connections with other sciences.
CLO 3. Inquiry and problem-solving:
3.1. You will be able to critically analyse and solve problems in biotechnology by gathering,
synthesizing and critically evaluating information from a range of sources.
3.2. You will be able to critically analyse and solve problems in biotechnology by designing
and planning an investigation, including developing a testable hypothesis.
3.3. You will be able to critically analyse and solve problems in biotechnology by selecting and
applying practical and/or theoretical techniques with technical competence in conducting field,
laboratory-based, or virtual experiments.
3.4. You will be able to critically analyse and solve problems in biotechnology by collecting,
accurately recording, interpreting, and drawing conclusions from scientific data.
CLO 4. Communication: You will be an effective communicator of biological sciences by
effectively communicating scientific results, information, or arguments using a range of modes
(oral, written, visual) for a variety of purposes and audiences.
CLO 5. Personal and professional responsibility: You will be accountable for individual
learning and scientific work in biotechnology by being an independent and self-directed learner.

Performance descriptors: For HD XXXXXXXXXX%) you need to achieve excellently all tasks followingthe instructions and guidelines listed above

CriteriaRatingsPts
Understanding of molecular cloning techniques and their applications100.0 Pts50.0 Pts25.0 Pts0.0 Pts100
Answered 3 days After May 28, 2022

Solution

Preeti answered on May 29 2022
89 Votes
Generation of cut flowers (eg roses) with an extended shelf-life using genetic engineering technologies
Genetic Engineering is a process of manipulation of an organisms’ DNA makeup using Biotechnology. Nowadays, genetically modified crops especially the modification of cut flowers is used extensively in floral markets as it is beneficial for florists (Anderson and Walker, 2013). Gene silencing is widely used producing the plants or animals for their commercial use, in drug therapeutics, cancer research etc. The extended shelf-life of cut flowers benefits the florists. It can be achieved by delaying the senescence of plants. Plant hormones plays an essential role in their aging and senescence. Ethylene, a gaseous plant hormone, is responsible for the aging of flowers and ripening of fruits. Hence, if the ethylene producing pathway is blocked by knocking down the Ethylene Production Gene (EPG), the shelf-life of the cut flowers can be extended. This can only be achieved by gene silencing.
Gene silencing is a process that regulates the expression of a gene and leads to its inactivation. There are multiple check pints, where the expression of a gene can be checked and regulated. It is a mechanism through which cells can cease a large section of their chromosomal DNA. Mostly the silencing of DNA in the repetitive sequences of both plants and animals are seen using DNA methylation (Selker, 1999). Gene silencing can either occur during transcription or during translation. It has been studied naturally in a cell as a defence mechanism against the viruses. RNAi recognizes the dsRNA and cuts into smaller RNA fragments which is then silenced the translation of proteins. The gene silencing can occur through DNA methylation, using long non-coding RNAs, siRNAs, etc. There are two methods through which silencing of gene can be done in plants:
i) Transcriptional Gene Silencing (TGS)
ii) Post-transcriptional Gene Silencing (PTGS) (El-Sappah et al., 2021)
TGS is achieved in the nucleus part of the cell, where the transcription can be blocked by inhibiting the promoter region. It can be done by RNA-directed DNA methylation, transposon silencing, paramutation etc. (Vaucheret and Fagard, 2001).
PTGS occurs in the cytoplasm region of a cell, where the mRNA of a specific...
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