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Mary is the scientific consultant of a firm of investors interested in biotechnology. She is asked to evaluate the research plan of a small company producing engineered variants of the protease subtilisin from Bacillus subtilis used in detergents. The company plans to use Escherichia coli as the host for the expression of these variants. The investors Mary is representing ask her to prepare a report evaluating the feasibility of this approach.

Answered Same DayNov 14, 2019Swinburne University of Technology

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David answered on Nov 30 2019
111 Votes
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Evaluation of the Research Plan of a Small Company Producing Engineered Variants of the Protease Subtilisin from Bacillus Subtilis used in Detergents
1. Objective
    The objective or purpose of this report is to evaluate the research plan of a small company producing engineered variants of the protease subtilisin from Bacillus subtilis used in detergents. This objective is essential to increase knowledge and understanding how a small company can produce engineered variants of the protease subtilisin from Bacillus subtilis using Escherichia coli as the host for the expression of these variants (Segura et al., 2008). At this stage, the objective of this report would be evaluated in the form of feasibility of this approach to fulfill requirements of the scientific consultant of a firm of investors interested in biotechnology.
2. Introduction
Problem:    
The production of engineered variants of the protease subtilisin from Bacillus subtilis is the most important consideration in biotechnology to make it commercially in the business process. However, there is a problem associated with the protein, subtilisin. Subtilisin is specifically known as a non-specific protease that worked as the protein-digesting enzyme (Segura et al., 2008). It is initially obtained from Bacillus subtilis through biotechnology engineering process (Segura et al., 2008). The problem of this protein can be assumed as inactivation in the presence of oxidation of a methionine that is specifically close to the active site.
    It is the true fact that an enzyme is linked with a biocatalyst that reduces the activation energy effectively and efficiently. However, it is effective to increase the velocity of the biochemical reaction. This protein is a Serine protease that is obtained with the help of bacillus subtilis, bacteria. Subtilisin is associated with three amino acids that are part of the active site of this protein (Segura et al., 2008). These amino acids are the Aspartic acid (D)-32, Histidine (H)-64, and Serine (S)-221. Catalystic triad has made with these three amino acids. Therefore, the hydrolization of the proteins into the peptides usually occurs due to these three amino acids. Now, the mutant of the subtilisin is required to use in commercial products, basically in detergent and cosmetics. At this stage, it is also stated that the native of the subtilisin cannot be used in the case of these products because of the detergent inhibition.
Characteristics of the Protein:
    Subtilisin shows various characteristics in the invention of this protein that are improved characteristics. These characteristics can be determined as substrate affinity, catalytic efficiency, and catalytic activity. Apart from this, subtilisin also shows characteristics as stability when washing conditions occur as well as it also shows overall wash performance. It is now a useful additive in the context of cleaning compositions (Kumar, Savitri , Thakur, Verma & Bhalla, 2008). This protein is also characterized as distinctive structure that usually consists of two beta-ba
el domains. This domain specifically converges on the position of the catalytic active site. The characteristic as catalytic mechanism of the subtilisin is used as a catalytic triad that helps to create a nucleophilic serine.
Reason of Production of the Protein:
    There are various reasons behind the production of the subtilisin protein, such as participation in physiological activities and regulation of gene expression. At the same time, the protein is being produced because this protein represented as one of the major groups of industrial enzymes. There are a number of detergent production companies that required such type of protein to use in detergents. This protein is worthwhile in the commercial activities in which the biotechnology engineering screened microbes from new habitats for subtilisin that has novel properties in order to meet the needs of rapidly growing detergent industry in the market (Kumar, Savitri , Thakur, Verma & Bhalla, 2008). The subtilisin is being produced because it is a high-alkaline protein that has been successfully applied to degrade the components of detergent formulations. In order to improve stability and performance of the detergent industry, the extensive protein engineering efforts produce subtilisin.
Impact on the Strategy Employed:
    The production of the subtilisin can impact on the strategy employed on the basis of fulfilling total demands of this protein to the different industry. However, detergent industry mostly requires subtilisin to increase capability of the product and wash performance (Wipat & Harwood, 2000). The production of subtilisin may also impact on the strategy employed by the organization through supporting a level of production that continuously produces different types of detergent products for the customers. The detergent industry employs strategies to produce detergent equal to the average demand. Therefore, consistency and scheduling of production of the industry need to a
ange the required quantity to manage market demand appropriately. Therefore, the production of subtilisin must be according to the demand of the industry otherwise the performance would also be reduced, including reducing sales and...
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