Fraction A
Fraction B
Fraction C
Fraction D
26/10/2020 GROUP 12 - Lab Prac Assessment (ONLINE)
https:
mit.instructure.com/courses/62987/assignments/ XXXXXXXXXX/4
GROUP 12 - Lab Prac Assessment (ONLINE)
Due Tuesday by 23:59 Points 10 Submitting a file upload
Available 26 Oct at 9: XXXXXXXXXXOct at 23:59 1 day
Submit Assignment
COURSE NAME: Biochemistry and Molecular Biology 1
COURSE CODE: BIOL1177
ASSIGNMENT TITLE: Lab Practical Exam (ONLINE)
DUE DATE: 27-October-2020
Overview of Assignment
As previously mentioned, following Stage 4 restrictions, all on-campus learning activities
including outstanding practicals in BIOL1177 were paused. Since then we have been
ethinking how we can deliver these essential learning activities in different ways so you
can continue to study without delay.
While Stage 4 restrictions are temporary, RMIT is committed to providing students with
certainty so you make informed decisions regarding your study plans. As we progressively
transition activities back to campus, we will continue to make decisions based on
Government advice and our own campus assessments. Government restrictions may be
einstated at any time dependent on the advice of the Chief Health Officer.
As the final assessment for BIOL1177 is now delivered completely online, there will be an
alternative assessment for the Laboratory Practical Examination.
For those students who attended the laboratory practical examination you will be given the
opportunity to undertake this assessment. This means that the mark you received on you
face-to-face laboratory practical examination will be superseded by the mark you obtain
on this assignment. It is up to you if you wish to do undertake this assignment
Assignment Breakdown
You will be asked to construct a standard curve, determine appropriate dilutions of an
unknown sample, plot a graph (eg. Using Microsoft Excel or any other statistical package),
determine the final concentration of the unknown protein and then comment on your results
It is recommended you read about the unknown dilution examples presented at the start of
the practical manual
Taksh Patel
Taksh Patel
26/10/2020 GROUP 12 - Lab Prac Assessment (ONLINE)
https:
mit.instructure.com/courses/62987/assignments/ XXXXXXXXXX/4
BIOL1177 - LAB PRAC EXAM (ONLINE)
You will be provided with a Proforma which provides details on the practical assessment
and space is provided for you to complete tables, calculations, draw your graph and
comment on your results. Please read the instructions on the proforma carefully
DO NOT MODIFY the proforma
TYPE your answers in the space provided
You must conform to the page limit. If you go over the page limit you will be penalised
This assessment is worth 10% of your overall mark
This assessment will be open on Monday 26th of October and 9AM and will close on
Tuesday 27th of October at MIDNIGHT
Access your assignment here: BIOL XXXXXXXXXXLaboratory Practical Assessment
ONLINE 73 FINAL.docx
Learning Outcomes
1. Explain the central role of biochemistry and molecular biology within the health and
iomedical disciplines.
2. Describe and explain the principles and fundamental concepts of biochemistry and
molecular biology with a focus on analytical techniques, proteins, enzymes, Vitamins and
Trace Elements, DNA and RNA metabolism and gene regulation.
4. Problem solve, validate and interpret results of biochemical investigations.
RMIT Electronic Submission of work for assessment
I declare that in submitting all work for this assessment I have read, understood and agree to
the content and expectations of the assessment declaration
(https:
www.rmit.edu.au/students/student-essentials/assessment-and-
exams/assessment/assessment-declaration) .
Please see additional info on pdf: Accessible Powerpoints.pdf
Â
https:
mit.instructure.com/courses/62987/files/ XXXXXXXXXX/download?wrap=1
https:
www.rmit.edu.au/students/student-essentials/assessment-and-exams/assessment/assessment-declaration
https:
mit.instructure.com/courses/62987/files/9864302/download?wrap=1
26/10/2020 GROUP 12 - Lab Prac Assessment (ONLINE)
https:
mit.instructure.com/courses/62987/assignments/ XXXXXXXXXX/4
Criteria Ratings Pts
0.5 pts
0.5 pts
0.5 pts
1.0 pts
0.5 pts
3.0 pts
0.5 pts
Aim
2-3
Sentences
0.5 Pts
Exemplary
Aims: A clear and concise description of what
the experiment is trying to achieve should be
presented. The following keywords should be
mentioned: cali
ations/standard curve,
quantify, Bradford, unknown albumin.
0.0 Pts
No marks
Aims: A clear and concise description of what the
experiment is trying to achieve is unclear o
missing. The following keywords should be
mentioned: cali
ations/standard curve, quantify,
Bradford, unknown albumin are missing.
PART 1.
Point 5.
Example
Calculation
0.5 Pts
Exemplary
The formula C1V1=C2V2 has co
ectly
een applied (formula and all working
out) for a selected tube from Table 1.
0.0 Pts
No marks
The formula C1V1=C2V2 has been applied (formula and
all working out) for a selected tube from Table 1 but some
or the majority of details are inco
ect or missing.
TABLE 1 0.5 Pts
Exemplary
Table 1 is complete and co
ect (volumes of
standard, volume of water, final volume, and final
concentration and co
ected abso
ance) has
co
ectly been presented. An appropriate table
caption has been included.
0.0 Pts
No marks
Table 1 is incomplete with either (volumes of
standard, volume of water, final volume, and
final concentration and co
ected abso
ance)
missing or incomplete. An appropriate table
caption has not been included.
PART 2.
Point 3.
1.0 Pts
Exemplary
Appropriate and feasible dilution
factors have co
ectly been
applied (including working out)
with a detailed explanation of why
these are required.
0.5 Pts
Accomplished
Appropriate and feasible dilution
factors have been applied (including
working out) with an explanation of
why these are required but some
minor e
ors are present.
0.0 Pts
No marks
Dilution factors have
een applied with a
limited or no
explanation of why
these are required.
TABLE 2 0.5 Pts
Exemplary
Table 2 is complete and co
ect (volumes of
unknown, dilution factors, dilution volumes,
co
ected abso
ance, unknown protein
concentration, and mean protein concentration)
has co
ectly been presented. An appropriate
table caption has been included.
0.0 Pts
No marks
Table 2 is incomplete with either (volumes of
unknown, dilution factors, dilution volumes,
co
ected abso
ance, unknown protein
concentration, and mean protein concentration)
missing or incomplete. An appropriate table
caption has not been included.
PART 4.
Bradford
Standard
Curve
3.0 Pts
Exemplary
Graph (Bradford
standard curve) has
clearly labelled
axes and units,
duplicate values
plotted, line of best
fit and a
appropriate figure
caption has been
included.
2.0 Pts
Accomplished
Graph (Bradford
standard curve) has
clearly labelled axes and
units, duplicate values
plotted, line of best fit
and a appropriate figure
caption has been
included but some
minor e
ors are present.
1.0 Pts
Developing
Graph (Bradford
standard curve) has
clearly labelled axes
and units, duplicate
values plotted, line of
est fit and a
appropriate figure
caption has been
included but majo
e
ors are present.
0.0 Pts
No marks
Graph (Bradford
standard curve) has
een inco
ectly
graphed labels and
axes, duplicate
values, line of best fit
and a appropriate
figure caption are
missing o
incomplete.
PART 4.
Point 2.
0.5 Pts
Exemplary
The concentrations of the unknown protein
samples (Neat, Dilution 1 and 2) (Tubes 13 -
18) have been drawn and labelled on the
graph.
0.0 Pts
No marks
The concentrations of the unknown protein samples
(Neat, Dilution 1 and 2) (Tubes XXXXXXXXXXhave
inco
ectly or not been drawn and labelled on the
graph.
26/10/2020 GROUP 12 - Lab Prac Assessment (ONLINE)
https:
mit.instructure.com/courses/62987/assignments/ XXXXXXXXXX/4
Total points: 10.0
Criteria Ratings Pts
2.0 pts
1.0 pts
0.5 pts
PART 4.
Point 3
2.0 Pts
Exemplary
The final protein
concentration
(including
calculations) is
co
ect. A detailed
discussion as to
which dilution was
used is well
justified.
1.5 Pts
Accomplished
The final protein
concentration
(including calculations)
has some minor e
ors
presented. A detailed
discussion as to which
dilution was used is
easonably justified.
1.0 Pts
Developing
The final protein
concentration (including
calculations) has some
major e
ors presented.
A detailed discussion
as to which dilution was
used is satisfactorily
justified.
0.0 Pts
No marks
The final protein
concentration
(including calculations)
is incomplete o
inco
ect. A detailed
discussion as to which
dilution was used is
missing or incomplete.PART 4.
Point 4
1.0 Pts
Exemplary
A discussion on the
concept of linearity and
associated e
ors have
een interpreted
co
ectly, demonstrating
an excellent
understanding of results
conveyed.
0.5 Pts
Accomplished
A discussion on the
concept of linearity and
associated e
ors have
een presented,
demonstrating an
adequate
understanding of
esults conveyed.
0.25 Pts
Developing
A discussion on
the concept of
linearity and
associated e
ors
have been
presented but
majo
improvments are
equired.
0.0 Pts
No marks
Incomplete o
inco
ect
interpretation of the
concept of linearity
and associated
e
ors, indicating a
complete lack of
understanding.Conclusion 0.5 Pts
Exemplary
A single paragraph which clearly and concisely
describes the outcome(s) of this experiment
has been presented.
0.0 Pts
No marks
A single paragraph which clearly and concisely
describes the outcome(s) of this experiment is
missing or incomplete.
Quantitation of human albumin in a patient’s plasma sample
Student Name: _________________
GROUP NUMBER: ______________________
Student Number: ______________________
Demonstrator: _________________________
Aim:________________________________________________________________________________________ __________________________________________________________________________________________________________________________________________________________________________________________
(0.5 mark)
Part 1. Prepare your standard curve.
1. You are provided with a standard albumin protein solution (200 ug/mL).
2. Create a 6-point standard curve from 0 ug/mL – 50 ug/mL. Each standard should be performed in duplicate (tubes XXXXXXXXXXAbso
ance values are provided below).
3. The final volume in each tube should be 0.5 mL.
4. Determine and record the final concentration and volume for each tube in Table 1below (include caption).
5. Provide an example calculation for one of your tubes by applying the formula C1V1=C2V XXXXXXXXXXbelow. Ensure you show all working out.
_____________________________________________________________________________________________________________________________________________________________________________ (0.5 mark)
______________________________________________________________________________________________________________________________________________________________________________________________
Table 1. ________________________________________________________________________________
Tube
1
2
3
4
5
6
Standard albumin (200 µg/mL), mL
H2O, µL
Final volume/tube, µL
Final [albumin] (µg/mL)
Abso
ance (595 nm)
0.402
0.424
0.568
0.560
0.661
0.658
Abso
ance - Blank
Tube
7
8
9
10
11
12
Standard albumin (200 µg/mL), mL
H2O, µL
Final volume/tube, µL
Final [albumin] (µg/mL)
Abso
ance (595 nm)
0.771
0.770
0.877
0.881
0.980
0.955
Abso
ance - Blank
(0.5 mark)
Part 2. Prepare your unknown patient’s plasma sample.
1. You are provided with an unknown protein solution containing albumin in the range 0 – 150 ug/mL.
2. You will need to perform 2 serial dilutions of the unknown albumin sample. You will need to prepare between 1 - 2 mL of the first dilution to be able to perform the next serial dilution.
3. Determine and record the appropriate dilution factors for both dilutions 1 and 2 in Table 2 below. Explain in detail why your unknown needs be diluted.
____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ___________________________________________________________________________________________
(1.0 mark)
4. Set up microfuge