Scoring for Lab 3:
Screen shot – 10 points
Activity Data Code – 5 points
Data Tables – 15 points
Question Completion – 20 points
Random Questions Scored – 50 points
· On your own and without assistance, complete this Lab Activity Form electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus).
· Answer ALL questions in your own words. If a direct quote is needed, put the words in quotation marks and cite the source.
· To conduct the laboratory exercises, use the Table Top Science link located under Course Content. Read the background information and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.
· Students should submit the document as a Word (.doc or .docx) or PDF file to the assignment folder for best compatibility.
· If screen shots are missing, the lab earns 0 points until it is included.
Data Sheet: Activity - Osmosis
All Content is Copyright Protected and May NOT Be Posted or Shared Outside Of The Classroom
Name
Course
Date
Activity Data Code
Take a screenshot of your results. Insert the picture here:
Procedure I - Test Solution 1: Water (Identical Concentrations)
Complete the tables and questions below using your data and information found under the Background ta
Data Table I Note: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Wate
Trial
Starting Volume of Test Solution
WATER
(L)
Starting Volume of Wate
(L)
Final Volume of Test
Solution
WATER
(L)
Final Volume of Wate
(L)
Difference
in Final Volumes
(L)
1
1.28
2
1.28
2
TableTop Science – All Rights Reserved
1
TableTop Science – All Rights Reserved
Observations and Questions
[1] A. Given that the final heights (and volumes) are the same for the water and test solution, what can you conclude about the amount of solutes in these two solutions?
B. Explain your reasoning. Include where water travels and why.
[2] As discussed in the Background material, water is an important biological macromolecule. A. Do you expect water to continue to flow across the semipermeable; lipid bilayer after osmotic equili
ium is reached?
B. Why or why not? Include where water travels and why.
Procedure II - Test Solution 2: Guanine solution – KNOWN Concentrations
Solution
Compound
mMolar Concentration
(mmol/L)
solution 2
guanine
0.170
Complete the tables and questions below using your data and information found under the Background ta
Data Table II Note: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Wate
2
TableTop Science – All Rights Reserved
3
TableTop Science – All Rights Reserved
Trial
Starting Volume of Test Solution
GUANINE
(L)
Starting Volume of Wate
(L)
Final Volume of Test Solution
GUANINE
(L)
Final Volume of Wate
(L)
Difference
in Final Volumes
(L)
1
1.28
2
1.28
Observations and Questions
[3] A. Why did the height (and volume) change in the test solution?
B. What is the basis for the increase in the volume in the test solution? Include where water travels and why.
[4] A. What do you think would happen if both starting volumes were the same?
B. Test your hypothesis by doing a data run. Explain your observation. Include where water travels and why.
[5] Why is the difference in final volumes the same for both trials? Include where water travels and why.
Procedure III - Test Solution 3: Cytochrome C solution – UNKNOWN concentrations
Solution
Compound
mMolar Concentration
(mmol/L)
solution 3
cytochrome c
to be determined
Complete the table and questions below using your data and information found under the Background tab (see the Summary of Needed Formulas section) and Activity Form ta
Data Table III Note: Difference in Final Volumes = Final Volume of Test Sol - Final Volume of Wate
4
TableTop Science – All Rights Reserved
5
TableTop Science – All Rights Reserved
Trial
Starting Volume of Test Solution
CYTOCHROME C
(L)
Starting Volume of Wate
(L)
Final Volume of Test Solution
CYTOCHROME C
(L)
Final Volume of Wate
(L)
Difference
in Final Volumes
(L)
1
1.28
2
1.28
Observations and Questions
[6] A. Based on your data and observations from procedure II and procedure III, which solution, has the highest concentration? Guinine or Cytochrome C?
B. Explain your answer. Include where water travels and why.
[7] Concentration Calculation: Using your Trial 1 data from procedure II and procedure III, calculate the concentration of the cytochrome c solution. Hint: See the Summary of Needed Formulas section under the Background tab and information found under the Activity Form tab. SHOW YOUR WORK
Concentration of Solution B = Difference in final volumes for solution B
Concentration of Solution A XXXXXXXXXXDifference in final volumes for solution A