From the following information determine the amino acid sequence of a peptide. N-terminal Edman...

Question

From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives three products 1. Arg 2. Peptide containing Ala, Lys 3. Peptide containing Asp, Met, Phe, Pro Mild Chymotrypsin cleavage gives 2 peptides 1. Peptide containing Asp, Pro 2. Peptide containing Ala, Arg, Lys, Met, Phe CNBr cleavage gives 2 peptides 1. Peptide containing Ala, Arg, Lys and homoserine 2. Peptide containing Asp, Phe, ProYou
 must supply the answer as the 3-letter amino acid sequence from 
N-terminus to C-terminus in the form (you must use dashes, not spaces 
between the amino acids) for example;Met-Thr-Glu-Trp

David · Accepted Answer

N-terminal degradation of the amino acid sequence of the peptide gives PTH-Alanine. Thus in 
there is Alanine in the N-terminal end of the peptide. 
There is no observable reaction with C terminal carboxypeptidase.C terminal carboxypeptidase 
A cleaves amino side of c-terminal amino acid except arginine, lysine, methionine and proline. 
As there is not any reaction with carboxypeptidase , then there must be any one of the mentioned 
amino acid in the c-terminal end.

From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives...

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