Consider the following data for E. coli B/r-pDW17 grown in a minimal medium supplemented with amino...

Question

Consider the following data for E. coli B/r-pDW17 grown in a minimal medium supplemented with amino acids. Estimate Dm and R. Compare the stability of this system to one with a glucose-minimal medium (Example XXXXXXXXXXIt has been claimed that gel immobilization stabilizes a plasmid-containing population. A factor suggested to be responsible for the stabilization is compartmentalization of the population into very small pockets. For example, the pocket may start with an individual cell and grow to a level of 200 cells per cavity. Develop a mathematical formula to compare the number of plasmid-free cells in a gel to that in a large, well-mixed tank.

Dr Shweta · Accepted Answer

Solution
Ans 1. Given: In the given question, the E. coli B/r-pDW17 was grown in the minimal medium supplemented with different amino acids and the value of generations at different time intervals and f- is given.
To find out: δμ and R
Solution: To calculate the value of δμ, firstly we’ll calculate lnf- for both the Values of D i.e., D = 0.3h-1 and 0.67 h-1as shown below:
	D = 0.3h-1
	D = 0.67h-1
	Time, h
	f-
	lnf
	Time, h
	f-
	lnf
	0
	0.003
	-5.80914
	0
	0.003
	-5.80914299
	4.6
	0.01
	-4.60517
	5.2
	0.01
	-4.60517019
	11.5
	0.04
	-3.21888
	10.3
	0.015
	-4.19970508
	16
	0.08
	-2.52573
	15.5
	0.05
	-2.99573227
	23.1
	0.3
	-1.20397
	20.6
	0.13
	-2.04022083
	27.7
	0.

Consider the following data for E. coli B/r-pDW17 grown in a minimal medium supplemented with amino acids. Estimate Dm and R. Compare the stability of this system to one with a glucose-minimal medium...

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