Assume you scale up from 1 l of 1 x 1010 cells/ml of 100% plasmid-containing cells to 20,000 l of 5...

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Assume you scale up from 1 l of 1 x 1010 cells/ml of 100% plasmid-containing cells to 20,000 l of 5 x 109 cells/ml, at which point overproduction of the target protein is induced. You harvest six hours after induction. The value of P is XXXXXXXXXXBefore induction µ+ = 0.95 h-1 and µ- = 1.0 h-1 . After induction µ+ is 0.15 h-1 . What is the fraction of plasmidcontaining cells at induction? What is the fraction of plasmid-containing cells at harvest?

Aditi · Accepted Answer

ANSWER
Given data: µ+ = 0.95 n-1
	       µ- = 1.0 n-1
                  P = 0.0005
a. To calculate no,
         no = (1 * 103) (1 * 1010) (1)
         no = 1013 cells
To calculate nfinal,
         	         nfinal = (1 * 103) (5 * 109) (2*104) = 1017 cells
Therefore,

Assume you scale up from 1 l of 1 x 1010 cells/ml of 100% plasmid-containing cells to 20,000 l of 5 x 109 cells/ml, at which point overproduction of the target protein is induced. You harvest six...

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