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Metabolic Biochemistry GST and the Effects of Curcumin Practical: Criterion-Referenced Marking Criteria Rubric You will be required to present class data in a laboratory report. In the week following...

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Metabolic Biochemistry GST and the Effects of Curcumin Practical: Criterion-Referenced Marking Criteria Ru
ic
You will be required to present class data in a laboratory report. In the week following the practical session there will be a tutorial
covering elements of the report write up. Report submission will occur the week after the tutorial.


The report
demonstrates an
understanding of the
scientific concepts
associated with the
topic being studied.
Weight 20%.
The report is
presented as
specified in the
provided
guidelines within
the Subject
Manual. Weight
20%.
The laboratory
esults are
presented in an
appropriate manner.
Weight 5%.
References are
cited in the
prescribed
method (i.e.
within the text
and in the
eference list).
Weight 5%.
The statistical
analyses have been
performed co
ectly.
Weight 10%.
The information
within the report has
een presented in a
coherent and concise
manner that is clear
to the reader. Weight
10%.
Students have
critically analysed the
experimental results
and associated
literature to draw
easoned conclusions
from the data. Weight
30%.
Disciplinary knowledge
Criterion High distinction Distinction Credit Pass Fail
The report demonstrates an
understanding of the scientific
concepts associated with the topic
eing studied. Weight 20%.
 Outstanding report overall.
 Very well written
introduction.
 Concisely covers the function
of GST, previous studies with
curcumin, why curcumin
might be significant and can
describe the tissue
localisation of GST.
 Includes aims that are
appropriately written in
concise scientific manner.
 Coherently and logically links
the data to our
understanding of the subject
matter. Very well referenced.
 Excellent report
overall.
 Well written
introduction.
 Covers GST and
curcumin.
 Includes well written
aims.
 Discussion is
comprehensive but
does not draw on the
literature much.
 Some minor
eferencing
inconsistencies.
 Very good report
overall.
 Introduction does not
sufficiently (in detail)
cover GST and
curcumin.
 Aims are not included
or described poorly.
 Discussion is
ief and
does not cover all the
esults.
 There is little
literature used
throughout the
eport.
 Referencing is
minimal
 Good report
overall.
 Introduction is
ief and does
not cover all the
main points
covered in this
practical.
 Written without
an
understanding
the concepts.
 Some
misconceptions
about the
experimental
aims are evident.
 Discussion is very
ief and shows
some lack of
understanding of
the results.
 Little effort to
draw upon the
literature.
 Not a good
eport that is
missing vital
information.
 Introduction
does not cover
GST or
curcumin.
 Introduction is
too
ief.
 Poorly written
with a clear
lack of
understanding
of the
experimental
aims.
 Parts of the
esults are
missing.
 Discussion is
too
ief and
with little
eference to
the results or
literature.
Professional skills
Criterion High distinction Distinction Credit Pass Fail
The report is presented as
specified in the provided
guidelines within the Subject
Manual. Weight 20%.

NB: Margins are to be at least
2.5cm top, bottom, left and right.
Font is to be Times New Roman 12
point or Ariel 11 point.
 The cover sheet and
declaration have been
completed.
 Kept within the specified
page limit.
 Table titles have been
included above the tables.
 Figure legends have been
included below the figures
and are understandable in
isolation of text.
 Used co
ect axis labels with
appropriate units.
 E
or bars included on the
graph are representative of
experimental standard
deviation.
 Professional presentation
with great attention to detail.
 Significant p-values indicated
with an asterisk in the table
and/or on the graph.
 Fonts used are appropriate
 Margins are within limits
 The cover sheet and
declaration have been
completed.
 Like HD level but
lacking in some aspects
of the presentation.
 Adequate and co
ect
presentation of figures
and tables.
 Figure legends and
table titles not totally
descriptive.
 Graph axes labelled
inappropriately.
 References
appropriately with only
minor referencing
issues.
 Fonts used are
appropriate
 Margins are within
limits
 Cover sheet and
declaration have been
completed.
 Kept within the page
limit.
 Table & figure titles
not in the co
ect
position.
 Figure legend/caption
not present or
inaccurate.
 Axis titles not
descriptive or without
units.
 E
or bars on the
graph are excel
generated (not
epresentative of
experimental
standard deviation).
 Fonts used are
inappropriate
 Margins are not
within limits
 Have not, or only
partially,
completed the
assignment
cover sheet and
declaration.
 Used excel to
label graphs and
placed title in the
wrong position.
 Table titles in the
wrong position.
 Titles of figures
and tables read
like sentences
ather than
descriptive titles.
 Figure axes not
labelled
adequately.
 Some
inconsistencies
in referencing
(either within
the text and/or
the reference
list).
 Have not
completed the
assignment
cover sheet
and
declaration.
 Figures or
tables not
included.
 Missing figures,
tables or axis
titles in graphs.
 Missing e
or
ars on graphs.
 Exceeded the
page limit.
 Have not used
any references.
The laboratory results are
presented in an appropriate
manner. Weight 5%.

https:
www.nature.com/scitable
topicpage/effective-writing-
XXXXXXXXXX
 Good use of the impersonal
tense (ie didn’t use “I”, “we”
“our” in sentences at all)
 Appropriate tenses used
(typically past tense)

 Used the word “we”
once or twice
 Tenses good
 Used the word “I”
once or twice
 Tenses good
 Riddled with
personal
eferences
 Tenses good
 Riddled with
personal
eferences
 Tenses all over
the place.
https:
www.nature.com/scitable/topicpage/effective-writing XXXXXXXXXX
https:
www.nature.com/scitable/topicpage/effective-writing XXXXXXXXXX
https:
www.nature.com/scitable/topicpage/effective-writing XXXXXXXXXX
References are cited in the
prescribed method (i.e. within the
text and in the reference list).
Weight 5%.
 Used appropriate
eferencing style in text and
for reference list.
 Slight inconsistencies
in referencing.
 Used referencing
style in text but made
a few e
ors in the
eference list.
 OR vice versa.
 Used
eferencing but
included author
initials in the ‘in
text’ citation.
 Several e
ors in
eferencing
made.
 Missing
eferences in
eference list.
 Referenced poor
sources (eg
Wikipedia, text
ooks)


 Did not use
appropriate
eferencing
style in text
and/or for
eference list.
 Inco
ectly
eferenced
journal
articles.
 No references
The statistical analyses have been
performed co
ectly. Weight 10%.
 Co
ect t-test
calculations
 Co
ect p-values.
 Co
ect presentation of
p-values in scientific
notation OR, simply
p<0.05.
 Co
ect explanation of
values being either
significantly higher or
lower and not just
‘significantly different’.
 Have taken note of the
egression analyses
provided and justified
the exclusion of outliers
accordingly.



 T-test performed
co
ectly.
 Not adequate
explanation of values
eing either
significantly higher or
lower.

 Co
ect t-test results .
 Co
ect p-values.
 Not presented in
scientific notation or
p<0.05.
 No (or inco
ect)
explanation of values
eing either
significantly higher or
lower.
 Did not take note of
the regression
analyses and did not
exclude outlying
data.
 Statistics
performed but
p-values are
inaccurate.
 Statistical
analysis
incomplete.
 No statistical
analysis
performed
Communication skills
Criterion High distinction Distinction Credit Pass Fail
The information within the
eport has been presented
in a coherent and concise
manner that is clear to the
eader. Weight 10%.
 Easy to read.
 Only requires one read of
each section to be fully
understood.
 To the point (concise, not
waffley). Excellent sentence
structure and development
of argument (story line).
 Excellent and clear
figures/tables (co
ect axis
titles etc) that are
understandable without
eading the text.
 Well written but
not outstanding
argument present.
 Good
tables/figures.
 Minor grammatical
or typographical
e
ors.
 Requires work to
understand the flow
of the argument
(story).
 Ve
ose, or skirts the
issue.
 Adequate
figures/tables but not
easily understood.
 Absence of axis titles
or legends in figures
and tables.
 Requires more than
one read to
understand the
concepts presented.
 Repetitive
 Base level writing.
 Little development
of argument.
 Ve
ose or too
ief.
 Poor figures and
tables.
 Writing is not clear
and contains some
grammatical e
ors.
 Poor sentence
structure.
 Lack of flow to the
argument
Answered Same Day May 14, 2020

Solution

Mayank answered on May 17 2020
132 Votes
Effect of Curcumin on GST specific enzyme using liver and kidney tissue sample solution
Abstract
The present study aimed to evaluate the role of curcumin as anticancer agent. Its activity was tested against GST specific activity using liver and kidney tissues.
The solutions of cytosol and mitochondria was taken from liver and kidney tissues as the test solution for curcumin. Standard spectroscopic methods were used for evaluation of the GST specific activity in four groups. Statistical analysis was performed using means with standard deviation (mean ± SD) values. For multiple group comparison, one-way analysis of variance (ANOVA) and Student’s t-test was used followed by post-hoc analysis using Dunnett’s test was used and significant values were set at the level of p≤0.05.
The results showed that curcumin has significant effect on cytosol as compared with mitochondria in both the tissues. The GST specific activity in terms of U/mg of protein and in liver cytosol group was significantly reduced by 79.24%, while kidney cytosol showed 80.80%, and in kidney mitochondria the activity was slightly reduced by 21.12% in presence of curcumin as compared without curcumin solutions.
This data suggested that curcumin has significant anti-cancer activity along with antioxidant activity using specific enzyme inhibition mechanisms.
Introduction
In developing and developed countries, cancer mortality cases have been risen throughout the World and is considered as one of the most leading cause of death in some of the Western countries. This alarming clinical data has continuously increasing the research activities in order to search some complementary and alternative medicine (CAM) approach against cancer and would work as cancer chemo preventive agents, mostly some substance which are mostly included the diet. Turmeric, the dried rhizome was one of the best example to work as chemo protective agent. Curcumin is one of the molecule in the World, which have ‘n’ number of biological activities and research has been continued to identify the mechanism behind the same. Turmeric (diferuloylmethane), a major yellow product obtained from the rhizome of Curcuma longa Linn (Salem, Rohani and Gillies, 2014). Plant is found to have significant protein kinase C inhibition activity, anti-inflammatory, and antioxidant properties. Besides, it has been widely used as a coloring agent in foods, drugs and cosmetics. Approximately, turmeric was used as 4 g/adult/day in various countries, which is one of the reason of low rate of colorectal cancer. It contain variety of curcuminoids and essential oils, which have highest biological activity. It has been reported to inhibit tumor formation in duodenum, skin, forestomach, and mice colon, rat sebaceous glands, tongue, colon, and in mammary glands (Gupta et al., 2012). It worked through various mechanism such as kinases inhibition, activity of transcription factors inhibition like nuclear factor-κB and activator protein-1, and destruction of expression of the enzyme (PERRONE et al., 2015).
Glutathione S-transferases (GSTs) also called as ligandins, they ate the group of phase II metabolic isozymes include in eukaryotic and prokaryotic. GST are best reported to have their activity as detoxification, by catalyzing the conjugation of the reduced form of glutathione (GSH) into xenobiotic substrates. Cytosolic, mitochondrial, and microsomal are the basic three subfamilies of GST, and they all are extremely diverse in most of the amino acid sequence. It significantly detoxifies most of the endogenous compounds like peroxidised lipids, drugs, pesticides, carcinogens, he
icides, and variably-derived epoxides and enables the
eakdown of xenobiotics, which defines to role in cancer. Cytosolic GSTP1-1, one of the GST family member isozyme has significant role in in cancer development and chemotherapeutic resistance. GSTP...
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