Experiment 3Part (1) Materials Rᶠ Acetaminophen (AC)“iodine stained” Rᶠ = 0.46...

Question

Experiment 3Part (1)    Materials    Rᶠ    Acetaminophen (AC)“iodine stained”    Rᶠ = 0.46     Acetanilide (Aa)    Rᶠ = 0,67    Caffeine (Cf)    Rᶠ = 0.13    Phenacetin (Ph)    Rᶠ = 0.6    Salicylamide (SA)    Rᶠ = 0,8part 2       Materials     unknown    Single     Caffeine (Cf)    Mixture    Caffeine (Cf), Acetaminophen (AC). Acetanilide

Robert · Accepted Answer

INDENTIFICATION OF ANALGESIC IN THE MEDICINE USING TLC 
ABSTRACT:  
Chromatography (chromous = colour) methods are used widely in organic chemistry 
laboratories for routine analysis. TLC is generally used to check the purity of a 
sample and to analyze the actual components of a mixture and also to follow the 
pace of reactions. Analgesics are a category of drugs, which show the general 
property of alleviating pain, can in patients. Some examples are acetaminophen, 
aspirin, ibrufen, diclofenac, methylsalicylate, caffeine etc. The active ingredient of the 
analgesic in the pain formula can be found out using TLC. In this experiment, TLC is 
performed on a mixture of analgesics, in a view to find out the exact components 
present in it. 
INTRODUCTION 
Chromatography can be used to separate mixtures of substances into its 
components. There are many form of chromatography and they all work with the 
same principle. They all have a stationary phase and a mobile phase. The mobile 
phase flows through the stationary phase and carries the components of the mixture 
with it. Different components travel at different rates. We'll look at the reasons for this 
further down the page. Thin layer chromatography is done exactly as it name 
denotes - using a thin, uniform layer of silica gel or alumina coated onto a piece of 
glass, plastic etc. The silica gel is the stationary phase. The mobile phase is a 
suitable liquid solvent or mixture of solvents. 
MATERIALS AND METHODS 
1. Standardisation of Rf values 
For performing TLC, commercially available precoated TLC plates were used. 2% 
solutions of acetaminophen, acetanilide, phenacetin, caffeine and  salicylamide were 
used to perform TLC. The purpose of this activity was to determine and standardise 
the Rf values of these compounds. A TLC plate was of approximate size 10cm x 7.6 
cm is used for the elution. Using a pencil and ruler,

Experiment 3 Part (1) Materials Rᶠ Acetaminophen (AC) “iodine stained” Rᶠ = 0.46 Acetanilide (Aa) Rᶠ = 0,67 Caffeine (Cf) Rᶠ = 0.13 Phenacetin (Ph) Rᶠ = 0.6 Salicylamide (SA) Rᶠ = 0,8 part 2 Materials...

Solution

Answer To This Question Is Available To Download

Related Questions & Answers

Submit New Assignment