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Details of requirements can be found in the unit laboratory manual. A copy of the marking guide used to mark the Lab Report can be located in the laboratory manual and on the unit Moodle site....

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Details of requirements can be found in the unit laboratory manual. A copy of the marking guide used to mark the Lab Report can be located in the laboratory manual and on the unit Moodle site. Students should refer to this when preparing for, and writing, their Lab Report.
The report must be submitted online through Moodle by 4pm, Thursday, Week 10. The maximum word limit for the Lab Report is 1450. The report will include the following sections, with suggested maximum words in parentheses: Introduction (suggested maximum of 400 words), Materials and Methods (suggested maximum 100 words), Results (suggested maximum 400 words, not including figures and tables), Discussion (suggested maximum 400 words), and Conclusion (suggested maximum 150 words).
Introduction and Aims (suggestedmaximum of 400 words)
This section, written in present tense, contains background information relevant to the topic with the purpose of providing a context for the experiments performed. This introduction should 'set the scene' for the experiment and your later discussion.Make sure that you reference any information from its appropriate source such as journal articles. The last part of the introduction should also describe the aim(s) of the experiment.
Materials and Methods (suggestedmaximum of 100 words)
Written in present tense, this section should only reference the relevant pages of the laboratory manual. You do not need to rewrite the methods section. However, if there are any deviations from the method in the manual, these must be pointed out so that someone who read your report would be able to repeat the experiment.
'The material and methods used in this report are described in the laboratory manual (Ashby, R (2017),Laboratory Manual forBiochemistry and Biochemistry G, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, pp. 65-75).'
Results (suggestedmaximum of 400 words, not including figures, graphs and tables)
Written in past tense, here you will present your final results in a coherent and logical manner using appropriate tables and graphs. All figures must include a figure number, title and legend, which must be referred to in the text e.g. Figure 1, or Graph 2. A brief description should explain what is observed in each graph or figure. Note the important aspects of your results, but be careful not to discuss your results in this section.
All raw data, data analysis (including standard curves) and example calculations should be included in an appendix.
Requirement for tables (should be prepared in Microsoft Excel):
  • Include a table number and a descriptive title below each table, as well as a table legend.
  • Be consistent with your decimal places, think about significant figures!

Requirement for graphs (should be prepared in Microsoft Excel):
  • Have a figure number and an appropriate title below each graph with an appropriate figure legend/key.
  • All graphs should fill an A4 size page and should be drawn using Excel.
  • Label both axes, indicating what the variable for that axis is, as well as what units you are using.

Discussion (suggested maximum of 400 words)
Write this section in past tense. This is your interpretation of the results given what background information you have available. Do not “rehash" the introduction.
The following points should be addressed, in a logical and coherent manner:
  • Briefly, what does the literature suggest the results should be?
  • What were your results?
  • Are these results consistent with the literature? If not, why not? You should also include reasons for any imprecision or errors encountered.

Conclusions (suggested maximum of 150 words)
Summariseyourresults andyourconclusions. Did you achieve your aims? What were those aims again? Your conclusions should relate back to the aims of the experiment.
Appendix
The tabulated data of your raw results and calculations should appear here.
References
The Lab Report must be referenced appropriately (e.g. acknowledge where you obtained information from) through in-text citation, and then listed at the end in a reference list. You should use the Harvard (author-date) system.
Answered Same Day Dec 26, 2021

Solution

David answered on Dec 26 2021
117 Votes
Enzyme Pyrophosphatase
Introduction:
Enzyme an simpler catalysts evolved in this primitive world is having a major(or)
vital role in the existence and functioning of biological process (mechanism) in simple
efe
ed as energy saver by lowering activation energy of the molecules. Inorganic
Pyrophosphate (PPi) an important phosphoryl donor group in the primitive earth
[1]
. The
functionality of proton pumps, sodium pumps and kinases based bacterium is dependent on
PPi donor group. The enzyme used in PPi catalyses the reaction by paving a way for new
active site of "mini-mineral" an responsible active site influenced by structural water
molecules
[2]
. The modern enzyme activity can be designed based on the analysis of PPi
enzyme study (based on spectroscopy adsorption).
The analysis helps in the identification and classification of PPi normally Type I
PPase and Type 2 PPase, type I are found in E.Coli and eukaryotes while later are found only
in bacteria representing the evolution chain in two different perspectives. Type I involves
three Mn
2+
ions while Type 2 is influenced by four Mn
2+
ions. In the active sites of PPase
transition state among proteins are stabilised by metal ions out of 14 conserved residues
[1]
of
Type I PPase kcat are decreased by residues of two aspartic acid. The key protonating for the
phosphroyl group is water molecules where it acts as catalysis in the scaffold of protein
moeity.
Aim:
The objective of this experimental work is to analyse the enzyme activity in PPase
using initiator and terminator of the reaction sequence which are numerically computed and
analysed using spectroscopic data's an UV-vis technique is employed for our PPi analysis.
Materials and Methods:
The blank and enzyme initiated reaction are performed in analysis and the guidelines
are as below,
Materials required:
A UV based spectroscopy, micropipette, glass sti
er, glass curvette (six numbers), microfi
e
cloth, acetone and DI water
The acetone are used in prior for removal of dirt and organic deposits for precise
eading from the experiment. Total of four methods are employed for the analysis of PPi,
1. Phosphate standard curve
2. Reaction velocity as a function of enzyme concentration
3. Reaction velocity as a function of pH
4. Enzyme activity as a function of Temperature
Methods:
1. Phosphate standard curve
i. Use micropipette in the preparation of standard blank samples, the PPi
concentration are computed and tabulated
ii. One blank sample near 0 mM of PPi are mixed with 2.5 ml...
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