Show your work. Consider the “reasonableness” of your final answer and do not forget to include units. You may not discuss this assignment with your peers.

**Circle your final answer.**

4.A 1:5 dilution of a 15 mg/mL glucose solution was made by the Medical Technologist. Once the dilution was made, the technologist transferred 1 mL of the solution into 4 mL of diluent. What is the final concentration of glucose in mg/dL?

5.You have a series of 5 tubes, each of which contain 4 mL diluent. You add 1 mL of sample to the first tube, mix and take 1 mL of this mixture and add to the second tube, mix and take 1 mL of this mixture and add to the next. If you continue this process with each tube, what is the dilution of the sample in the last tube?

6.You made a 1:2 dilution of serum. Then you added 4 mL of that to 8 mL of water. Then 1 mL of that was diluted to 8 mL. What was the final dilution of serum?

7.You took 8 mL of a glucose solution and diluted it to 20 mL. Then a 1:10 dilution was made of that. What was the final dilution?

8.In performing a serological procedure, you add 1 mL of serum to 10 mL of saline to tube

# 1. A 1:2 serial dilution is then made (starting from tube #2). What is the dilution of serum contained in tube 6?

- Describe schematically (draw a picture) how to make five sample dilutions from 1:2 to 1:32 so that each tube has a final volume of 0.1 mL and minimal sample is wasted.

- You need to prepare 1 dL of a 100 mg/mL solution of CuSO
_{4}. The available reagent is CuSO_{4}·5 H_{2}O. (CuSO_{4 }with 5 water molecules attached) How many grams of this hydrated reagent is needed to make 1 dL of a 100 mg/mL solution of CuSO_{4}?

- You need to make 2L of 0.2M acetic acid (CH
_{3}COOH). All you have available is concentrated glacial acetic acid (assay value, 99%; specific gravity, 1.05 g/mL). How many mL of the glacial acetic acid is needed to make this solution

4.A 1:5 dilution of a 15 mg/mL glucose solution was made by the Medical Technologist. Once the dilution was made, the technologist transferred 1 mL of the solution into 4 mL of diluent. What is the final concentration of glucose in mg/dL?

5.You have a series of 5 tubes, each of which contain 4 mL diluent. You add 1 mL of sample to the first tube, mix and take 1 mL of this mixture and add to the second tube, mix and take 1 mL of this mixture and add to the next. If you continue this process with each tube, what is the dilution of the sample in the last tube?

6.You made a 1:2 dilution of serum. Then you added 4 mL of that to 8 mL of water. Then 1 mL of that was diluted to 8 mL. What was the final dilution of serum?

7.You took 8 mL of a glucose solution and diluted it to 20 mL. Then a 1:10 dilution was made of that. What was the final dilution?

8.In performing a serological procedure, you add 1 mL of serum to 10 mL of saline to tube

# 1. A 1:2 serial dilution is then made (starting from tube #2). What is the dilution of serum contained in tube 6?

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