Show your work. Consider the “reasonableness” of your final answer and do not forget to include units. You may not discuss this assignment with your peers.Circle your final answer.
- Describe schematically (draw a picture) how to make five sample dilutions from 1:2 to 1:32 so that each tube has a final volume of 0.1 mL and minimal sample is wasted.
- You need to prepare 1 dL of a 100 mg/mL solution of CuSO4. The available reagent is CuSO4·5 H2O. (CuSO4 with 5 water molecules attached) How many grams of this hydrated reagent is needed to make 1 dL of a 100 mg/mL solution of CuSO4?
- You need to make 2L of 0.2M acetic acid (CH3COOH). All you have available is concentrated glacial acetic acid (assay value, 99%; specific gravity, 1.05 g/mL). How many mL of the glacial acetic acid is needed to make this solution
4.A 1:5 dilution of a 15 mg/mL glucose solution was made by the Medical Technologist. Once the dilution was made, the technologist transferred 1 mL of the solution into 4 mL of diluent. What is the final concentration of glucose in mg/dL?
5.You have a series of 5 tubes, each of which contain 4 mL diluent. You add 1 mL of sample to the first tube, mix and take 1 mL of this mixture and add to the second tube, mix and take 1 mL of this mixture and add to the next. If you continue this process with each tube, what is the dilution of the sample in the last tube?
6.You made a 1:2 dilution of serum. Then you added 4 mL of that to 8 mL of water. Then 1 mL of that was diluted to 8 mL. What was the final dilution of serum?
7.You took 8 mL of a glucose solution and diluted it to 20 mL. Then a 1:10 dilution was made of that. What was the final dilution?
8.In performing a serological procedure, you add 1 mL of serum to 10 mL of saline to tube
# 1. A 1:2 serial dilution is then made (starting from tube #2). What is the dilution of serum contained in tube 6?