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Show your work. Consider the “reasonableness” of your final answer and do not forget to include units. You may not discuss this assignment with your peers. Circle your final answer. Describe...

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Show your work. Consider the “reasonableness” of your final answer and do not forget to include units. You may not discuss this assignment with your peers.
Circle your final answer.
  1. Describe schematically (draw a picture) how to make five sample dilutions from 1:2 to 1:32 so that each tube has a final volume of 0.1 mL and minimal sample is wasted.

  1. You need to prepare 1 dL of a 100 mg/mL solution of CuSO4. The available reagent is CuSO4·5 H2O. (CuSO4 with 5 water molecules attached) How many grams of this hydrated reagent is needed to make 1 dL of a 100 mg/mL solution of CuSO4?

  1. You need to make 2L of 0.2M acetic acid (CH3COOH). All you have available is concentrated glacial acetic acid (assay value, 99%; specific gravity, 1.05 g/mL). How many mL of the glacial acetic acid is needed to make this solution

4.A 1:5 dilution of a 15 mg/mL glucose solution was made by the Medical Technologist. Once the dilution was made, the technologist transferred 1 mL of the solution into 4 mL of diluent. What is the final concentration of glucose in mg/dL?
5.You have a series of 5 tubes, each of which contain 4 mL diluent. You add 1 mL of sample to the first tube, mix and take 1 mL of this mixture and add to the second tube, mix and take 1 mL of this mixture and add to the next. If you continue this process with each tube, what is the dilution of the sample in the last tube?
6.You made a 1:2 dilution of serum. Then you added 4 mL of that to 8 mL of water. Then 1 mL of that was diluted to 8 mL. What was the final dilution of serum?
7.You took 8 mL of a glucose solution and diluted it to 20 mL. Then a 1:10 dilution was made of that. What was the final dilution?
8.In performing a serological procedure, you add 1 mL of serum to 10 mL of saline to tube
# 1. A 1:2 serial dilution is then made (starting from tube #2). What is the dilution of serum contained in tube 6?
Answered Same DayDec 27, 2021

Solution

Robert answered on Dec 27 2021
72 Votes
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